COXII | Plant Cytochrome oxidase subunit II (affinity purified) | AS04 053A

COXII | Plant Cytochrome oxidase subunit II (affinity purified) | AS04 053A | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conugated synthetic peptide fully conserved in all available protein sequences from eudicots including Arabidopsis thaliana AtmG00160, monocots including Oryza sativa P04373 and Physcomitrella patens Q1XGA9

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Blue Native-PAGE (BN-PAGE), Western blot (WB)

Related Products: AS04 052 | Anti-COXII (plant), chicken antibodiesAS04 053A-200 | Anti-COXII | cytochrome oxidase subunit II (plant), rabbit antibodiesAS04 053P COXII | cytochrome oxidase subunit II | Blocking peptide Antibodies to other mitochondrial proteinsPlant protein extraction buffer

Recommended Dilutions: 1 : 1000 (BN-PAGE), 1 : 1000 (WB)

Molecular weight: 29.4 | 30 kDa (for Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana (leaf extract and isolated mitochondria), Betula nana, Brassica napus, Brassica oleracea, Cicer aretinum, Cucumis melo, Cucumis sativus, Erophorum vaginatum, Hordeum vulgare, Lilium longifolorum, Nicotiana tabacum, Picea abies, Plantago major, Plantago euryphylla, Silene uniflora, Silene dioica, Physcomitrium patens, Triticum aestivum, Triticum durum Desf., Zea mays, Vicia faba, Quercus rubra

Predicted Reactivity: Cucumis melo, Glycine max, Oryza sativa, Physcomitrium patens, Pisum sativum, Triticum aestivum, Vigna radiataSpecies of your interest not listed? Contact us

Not reactive in: Saccharina japonica

Additional Information: Antibody detects COXII protein most optimally in membrane fractions. The signal is weak in a in total protein extract.Blue Native gel electrophoresis (BN-PAGE) has been performed on samples solubilized with digitonin (4:1) and loaded at 100 µg/well. Gel thickness was 2 mm with 4.5-16 % gradient.

Background: Cytochrome c oxidase (COX) catalyzes the reduction of oxygen to water in the respiratory chain in the inner mitochondrial membrane. Subunits 1-3 form the functional core of the enzyme complex. Subunit 2 (COXII) transfers the electrons from cytochrome c via its binuclear copper A center to the bimetallic center of the catalytic subunit 1. Alternative name: cytochrome c oxidase subunit 2

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: ATMG00160

Category: Mitochondria | Respiration

Research Area: Plant Biology