CRP ELISA kit | 55R-IB59126

CRP ELISA kit | 55R-IB59126 |

Activity Code: ACTIVE

Product Type: Research Kits

Product Subtype: Kits

Research Area: Hormones & Steroids

Short Description: ELISA kit for the detection of CRP in the research laboratory

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: N/A

Residues: N/A

Tag/conjugate: N/A

Protein Type: N/A

Expression System: N/A

Grade & Purity: N/A

Methode of Purification: N/A

Source: N/A

Concentration: N/A

Form & Buffer: N/A

Storage: Store at 2-8 deg C

Shipping Information: *Blue Ice*

Applications: ELISA

Bioactivity: N/A

Usage Recommendations: Optimal conditions to be determined by end user

Assay Information: The principle of this enzyme immunoassay test follows a typical twostep capture or ‘sandwich’ type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for CRP is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of CRP is conjugated to horse radish peroxidase (HRP) . CRP from the sample and standards are allowed to bind to the plate, washed, and  subsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. The  enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of CRP in the sample. A set of standards is used to plot a standard curve from which the amount of CRP in patient samples and controls can be directly read.