CSD2 | Chloroplastic Cu/Zn superoxide dismutase | AS06 170

CSD2 | Chloroplastic Cu/Zn superoxide dismutase | AS06 170 | Gentaur UK, US & Europe Distribution

Immunogen: overexpressed Arabidopsis thaliana Cu/ZnSOD O78310, At2g28190 with an N-terminal His-tag. Purified via Ni-column. The His-tag was cleaved and the protein was recovered via ion exchange on a 10 ml resource-Q (Pharmacia) column using a NaCl gradient. Pure fractions after dialysis against PBS has been used for immunization.

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

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Recommended Dilutions: 1 : 1000-1 : 4000 (WB)

Molecular weight: 22 | 19 kDa (Arabidopsis thaliana)

Confirmed Reactivity: Arabidopsis thaliana, Armeria maritima, Brassica juncea, Digitaria sanguinalis, Echinochloa crus-galli, Eragrostis tef, Impatiens walleriana, Iris pumila, Liquidambar formosana, Medicago sativa, Musa acuminate, Musa paradisiaca L., Nepeta cataria, Nepeta rtanjensis, Oryza sativa, Phalenopsis Sogo Yukidian cultivar V3, Phaseolus vulgaris, Pisum sativum, Populus sp., Schima superba, Solanum lycopersicum, Solanum tuberosum, Zea mays

Predicted Reactivity: Cucumis melo, Glycne max, Pinus pinasterSpecies of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Note: Antibody is recognizing 19 kDa protein Csd2 (chloroplastic enzyme) and gives also low but noticeable reactivity to Csd1 (15 kDa) a cytosolic form

Background: Antioxidant system works as a defense against oxidative stress. SOD (superoxide dismutase) catalyzes the dismutation of superoxide into oxygen and H202, . SODs are classified, according to their metal cofactor, as FeSOD, MnSOD, or Cu / ZnSOD. Chloroplasts generally contain Cu/ZnSOD and, in a number of plant species, FeSOD

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT2G28190

Category: microRNA targets

Research Area: RNAi/siRNA/miRNA