CTHRC1 Antibody | 8101

CTHRC1 Antibody | 8101 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: CTHRC1 antibody was raised against a 19 amino acid peptide near the amino terminus of human CTHRC1.
The immunogen is located within the first 50 amino acids of CTHRC1.

Research Area: Cancer

Tested Application: E, WB, IHC-P, IF

Application: CTHRC1 antibody can be used for detection of CTHRC1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: CTHRC1 antibody is human, mouse and rat reactive. At least two isoforms of CTHRC1 are known to exist; this antibody will detect only the larger isoform.

Positive Control 1: Cat. No. 1468 - Rat Small Intestine Tissue Lysate

Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 27 kDa
Observed: 27 kDa

Validation: N/A

Isoform: N/A

Purification: CTHRC1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CTHRC1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CTHRC1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Collagen triple helix repeat-containing protein 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The Collagen triple helix repeat-containing protein (CTHRC1) is a glycosylated, secreted protein that is transiently expressed in the arterial wall in response to injury where it is thought to contribute to vascular remodeling by inhibiting collagen expression and deposition and promoting cell migration (1) . CTHRC1 is highly expressed multiple human cancers, including pancreatic cancer where it plays a significant role in the progression and metastasis of the disease by regulating migration and adhesion of tumors cells (2) . CTHRC1 overexpression can also be used as an independent prognostic marker in gastric cancer (3) . Mutations at this locus have been associated with Barrett esophagus and esophageal adenocarcinoma (4) .