CX3CR1 Antibody | 2201

CX3CR1 Antibody | 2201 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Rat: (100%) , Rabbit: (93%) , Mouse: (93%) , Bovine: (86%)

Immunogen: CX3CR1 antibody was raised against a peptide corresponding to amino acids in a extracellular loop of human CX3CR1.
The immunogen is located within amino acids 160 - 210 of CX3CR1.

Research Area: Chemokines & Cytokines, Cancer

Tested Application: E, WB, IHC-P, IF

Application: CX3CR1 antibody can be used for detection of CX3CR1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of CX3CR1 are known to exist.

Positive Control 1: Cat. No. 1208 - THP-1 Cell Lysate

Positive Control 2: Cat. No. 1212 - 3T3 Cell Lysate

Positive Control 3: Cat. No. 17-008 - THP-1 Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 39, 43 kDa
Observed: 50 kDa

Validation: N/A

Isoform: N/A

Purification: CX3CR1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CX3CR1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CX3CR1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: CX3CR1 Antibody: V28, CCRL1, GPR13, CMKDR1, GPRV28, CMKBRL1, CX3C chemokine receptor 1, Beta chemokine receptor-like 1, C-X3-C CKR-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: CX3CR1 Antibody: CX3CR1 is one of the chemokine receptors that are required as coreceptors for HIV infection. The genes encoding human, mouse, and rat CX3CR1 were cloned and designated V28 and CMKBRL1, CX3CR1, and RBS11, respectively. The encoded seven transmembrane protein was initially identified as the receptor for the transmembrane molecule fractalkine, and renamed CX3CR1. CX3CR1 is known to serve as a coreceptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by fractokine. CX3CR1 mediates leukocyte migration and adhesion. CX3CR1 is expressed in a variety of human tissues and cell lines.