CXCR3 Antibody | 7647

CXCR3 Antibody | 7647 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Goat: (89%) , Bovine: (88%)

Immunogen: CXCR3 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human CXCR3 .
The immunogen is located within the last 50 amino acids of CXCR3.

Research Area: Chemokines & Cytokines

Tested Application: E, WB, ICC, IF

Application: CXCR3 antibody can be used for detection of CXCR3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: CXCR3 antibody is human, mouse and rat reactive. At least two isoforms of CXCR3 are known to exist; this antibody will detect both isoforms.

Positive Control 1: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 2: Cat. No. 17-011 - Hep G2 Cell Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 46 kDa
Observed: 43 kDa

Validation: N/A

Isoform: N/A

Purification: CXCR3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: CXCR3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: CXCR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: CXCR3 Antibody: GPR9, MigR, CD182, CD183, Mig-R, CKR-L2, CMKAR3, IP10-R, GPR9, C-X-C chemokine receptor type 3, CXC-R3

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The CXCR3 protein is a G protein-coupled receptor with selectivity for three chemokines, termed CXCL9/Mig (monokine induced by interferon-gamma) , CXCL10/IP10 (interferon-gamma-inducible 10 kDa protein) and CXCL11/I-TAC (interferon-inducible T cell alpha-chemoattractant) (reviewed in 1) . Binding of chemokines to this protein induces cellular responses that are involved in leukocyte traffic, most notably integrin activation, cytoskeletal changes and chemotactic migration (2) . Similar to other chemokine receptors such as CXCR4, CXCR3 has been implicated in several malignancies as a biomarker of tumor behavior as well as a potential therapeutic target (3) .