Cyclin B1 Antibody [V92.1] | 33-075

Cyclin B1 Antibody [V92.1] | 33-075 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human, Mouse, Hamster

Homology: N/A

Immunogen: Hamster protein was used as the immunogen for this Cyclin B1 antibody.

Research Area: Cancer, Cell Cycle

Tested Application: Flow, IP, IF

Application: Flow Cytometry: 0.5-1 ug/million cells
IF: 1-2 ug/ml
IP: 1-2ug/500ug protein--precipitates active Cyclin B1 complexes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Protein G affinity chromatography

Clonality: Monoclonal

Clone: V92.1

Isotype: IgG1, kappa

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide

Concentration: 0.2 mg/mL

Storage Condition: Aliquot and Store at 2-8˚C. Avoid freez-thaw cycles.

Alternate Name: G2/mitotic-specific cyclin-B1, CCNB1, CCNB

User Note: Optimal dilutions for each application to be determined by the researcher

BACKGROUND: This antibody recognizes a protein of 55-62kDa, identified as Cyclin B1. In mammals, Cyclin B1 associates with inactive p34cdc2, which facilitates phosphorylation of p34cdc2 at amino acids Thr-14 and Tyr-15. This maintains the inactive state until the end of G2-phase. The inactive Cyclin B1-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates amino acids Thr-14 and Tyr-15 of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B1 is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Cyclin B1-p34cdc2 plays a critical role in G2 to M transition.