Cyclin O Antibody | 3859

Cyclin O Antibody | 3859 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Cyclin O antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human Cyclin O.
The immunogen is located within the first 50 amino acids of Cyclin O.

Research Area: Apoptosis

Tested Application: E, WB

Application: Cyclin O antibody can be used for the detection of Cyclin O by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms.

Positive Control 1: Cat. No. 1410 - Mouse Bladder Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 39 kDa

Validation: N/A

Isoform: N/A

Purification: Cyclin O Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Cyclin O Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Cyclin O antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Cyclin O Antibody: CCNU, UDG2, Cyclin-O

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.