CYP1A2 Antibody [APG02872G]

CYP1A2 Antibody [APG02872G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: This CYP1A2 antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 255-282 amino acids from human CYP1A2.

Cellular Localisation: Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein

Molecular Weight: 58407

Clone: Monoclonal

Gene Name: CYP1A2 {ECO:0000303|PubMed:2575218, ECO:0000312|HGNC:HGNC:2596}

Gene ID: 1544

Function: A cytochrome P450 monooxygenase involved in the metabolism of various endogenous substrates, including fatty acids, steroid hormones and vitamins (PubMed:9435160, PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576) . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed:9435160, PubMed:10681376, PubMed:11555828, PubMed:12865317, PubMed:19965576) . Catalyzes the hydroxylation of carbon-hydrogen bonds (PubMed:11555828, PubMed:12865317) . Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta- estradiol (E2), namely 2-hydroxy E1 and E2 (PubMed:11555828, PubMed:12865317) . Metabolizes cholesterol toward 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis (PubMed:21576599) . May act as a major enzyme for all-trans retinoic acid biosynthesis in the liver. Catalyzes two successive oxidative transformation of all-trans retinol to all-trans retinal and then to the active form all-trans retinoic acid (PubMed:10681376) . Primarily catalyzes stereoselective epoxidation of the last double bond of polyunsaturated fatty acids (PUFA), displaying a strong preference for the (R, S) stereoisomer (PubMed:19965576) . Catalyzes bisallylic hydroxylation and omega-1 hydroxylation of PUFA (PubMed:9435160) . May also participate in eicosanoids metabolism by converting hydroperoxide species into oxo metabolites (lipoxygenase-like reaction, NADPH- independent) (PubMed:21068195) . Plays a role in the oxidative metabolism of xenobiotics. Catalyzes the N-hydroxylation of heterocyclic amines and the O-deethylation of phenacetin (PubMed:14725854) . Metabolizes caffeine via N3-demethylation (Probable) .

Summary: Tissue Location: Liver.

Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Euglobin precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB

Dilution: WB--1:500~1000

Synonyms: Cytochrome P450 1A2, CYPIA2, Cytochrome P (3) 450, Cytochrome P450 4, Cytochrome P450-P3, CYP1A2