Cystathionine Synthase, human recombinant | 7844

Cystathionine -synthase (CBS; E.C. 4.2.1.22) is a PLP-dependent enzyme which plays a central role in sulfur amino acid metabolism in eukaryotes. CBS catalyzes condensation between serine and homocysteine to generate cystathionine, which is then further processed by cystathionine -lyase to yield cysteine. The gene encoding CBS is essentially linked to the genetic disorders of homocystinuria and Down syndrome. Homocystinuria is an autosomal recessive disease, characterized by high plasma levels of homocysteine, with clinical manifestations including mental retardation, thromboembolism and connective tissue defects. In addition, CBS also mediates synthesis of hydrogen sulfide by catalyzing condensation between cysteine and homocysteine. CBS is highly expressed in the nervous system, liver and kidney and is responsible for up to 95% of the H2S production in the brain.

7844 | Cystathionine Synthase human recombinant DataSheet

Biomolecule/Target: Cystathionine Synthase

Synonyms: Beta-thionase, methylcysteine synthase, serine sulfhydrase

Alternates names: Fibroblast Growth Factor-20, FGFK

Taglines: 90% Pure Active Human recombinant Cystathionine Synthase, an enzyme involved in sulphur amino acid metabolism

NCBI Gene ID #: 26281

NCBI Gene Symbol: FGF20

Gene Source: Human

Accession #: Q9NP95

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): 100 U/mg

Biological activity: The ED as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is <10 ng/ml, corresponding to a specific activity of 1 x 10E5 units/mg.

Results: 100 U/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme required to convert 1.0 mole of L-homocysteine to cystathionine and hydrogen sulfide per minute in 200 mM Tris pH 8.6 at 37 °C

Molecular Weight: 23.2 kDa

Concentration: 0.5 mg/ml

Appearance: Liquid

Physical form description: 0.5 mg/ml in 50 mM Tris, 100 mM NaCl, pH 8.0 and 20% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A