Cytochrome B5A, human recombinant | 7871

Cytochrome b5a (CYB5A) is a membrane-bound hemoprotein which functions as an electron carrier for several membrane bound oxygenases like cytochrome P450 enzymes. Defects in the CYB5A gene (METHB-CYB5A) cause methemoglobinemia also known as CYB5A-related methemoglobinemia (a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of blood, cyanosis and hypoxia). ’s human recombinant CYB5A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-108 a.a of Uniprot Accession# P00167) and having a molecular mass of 14.9kDa. CYB5A is fused to a 24 amino acid long His-tag at the N-terminus & purified by proprietary chromatographic techniques.

7871 | Cytochrome B5A human recombinant DataSheet

Biomolecule/Target: Cytochrome B5A

Synonyms: Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5, Cytochrome B5

Alternates names: Fibroblast Growth Factor-20, FGFK

Taglines: 95% Pure Human recombinant Cytochrome B5A which can function as an electron carrier for Cytochrome P450 enzymes

NCBI Gene ID #: 26281

NCBI Gene Symbol: FGF20

Gene Source: Human

Accession #: Q9NP95

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: The ED as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF-receptors is <10 ng/ml, corresponding to a specific activity of 1 x 10E5 units/mg.

Results: N/A

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 23.2 kDa

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: Sterile filtered, 1 mg/ml solution containing 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 0.1 M NaCl and 1 mM DTT

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A