DAF-2 is highly sensitive reagent for NO detection and determination of nitric oxide synthase activity. DAF-2, however, remains essentially nonfluorescent until it reacts with the nitrosonium cation (produced by spontaneous oxidation of nitric oxide) to form a fluorescent heterocycle, which becomes trapped in the cell's cytoplasm. This sensitive fluorescent probe has been used to identify individual nitric oxide-producing neurons in brain slices, in mitochondria and in living plant cells.

Specifications

Purity

≥95% (HPLC)

Identity

1H-NMR

Appearance

Light yellow powder

Properties

Fluorescence

λex 505 nm, λem 518 nm in 0.1 M Tris pH 8.0

Solvents

DMSO