DAF Antibody | 7937

DAF Antibody | 7937 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: N/A

Immunogen: DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF.
The immunogen is located within amino acids 360 - 410 of DAF.

Research Area: Immunology

Tested Application: E, WB, IHC-P, IF

Application: DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.

Positive Control 1: Cat. No. 1203 - A549 Cell Lysate

Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide

Positive Control 3: Cat. No. 1201 – HeLa Cell Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 48 kDa
Observed: 49 kDa

Validation: N/A

Isoform: N/A

Purification: DAF antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DAF antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Decay-accelerating factor, CD55 molecule decay accelerating factor for complement (Cromer blood group) , CD55, CR, TC, CROM

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The decay-accelerating factor (DAF) , also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1) . DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2) . Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3) .