Description
DAF Antibody | 7937 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: DAF antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DAF.
The immunogen is located within amino acids 360 - 410 of DAF.
Research Area: Immunology
Tested Application: E, WB, IHC-P, IF
Application: DAF body can be used for detection of DAF by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: DAF antibody is human specific. At least two isoforms of DAF are known to exist; this antibody will only detect the longest isoform.
Positive Control 1: Cat. No. 1203 - A549 Cell Lysate
Positive Control 2: Cat. No. 10-101 - Human Lung Tissue Slide
Positive Control 3: Cat. No. 1201 – HeLa Cell Lysate
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 48 kDa
Observed: 49 kDa
Validation: N/A
Isoform: N/A
Purification: DAF antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: DAF antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: DAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: Decay-accelerating factor, CD55 molecule decay accelerating factor for complement (Cromer blood group) , CD55, CR, TC, CROM
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The decay-accelerating factor (DAF) , also known as CD55, is an integral membrane glycoprotein that is involved in the regulation of the complement cascade (1) . DAF limits excessive complement activation by accelerating their decay following DAF binding, thereby disrupting the cascade and preventing damage to host cells (2) . Antigens present on the DAF glycoprotein constitute the Cromer blood group system (CROM) (3) .