DAP Antibody | 7389

DAP Antibody | 7389 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (89%)

Immunogen: DAP antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DAP.
The immunogen is located within the last 50 amino acids of DAP.

Research Area: Autophagy

Tested Application: E, WB, IHC-P, IF

Application: DAP antibody can be used for detection of DAP by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: DAP antibody is human, mouse and rat reactive.

Positive Control 1: Cat. No. 1308 - Human Small Intestine Tissue Lysate

Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 11 kDa
Observed: 11 kDa

Validation: N/A

Isoform: N/A

Purification: DAP Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DAP Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: DAP Antibody: DAP1, Death-associated protein 1, DAP-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: DAP Antibody: The Death-associated protein (DAP) is a basic proline-rich 15kDa protein that as a positive mediator of programmed cell death that is induced by interferon-gamma (1) . DAP is also a direct substrate of mammalian target of rapamycin (mTOR) , a serine/threonine kinase that regulates cell growth and cell cycle, and a negative regulator of autophagy. Under rich nutrient conditions, mTOR phosphorylates DAP at Ser3 and Ser51; under starvation conditions, these residues are dephosphorylated and DAP is converted into an active suppressor of autophagy (2) .