DAPI | B1098

DAPI is a fluorescent, cell-permeable DNA-binding dye preferentially binds to DNA rich in adenine and thymine. DAPI is useful in measuring nuclear DNA content using flow cytometry for sorting isolated chromosomes and is also useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. DAPI can also be used to detect mycoplasma and in simple retrograde double-labelling procedures for studying axonal branching employing Evans Blue and Primulin.

DAPI is a fluorescent, cell-permeable DNA-binding dye preferentially binds to DNA rich in adenine and thymine. DAPI is useful in measuring nuclear DNA content using flow cytometry for sorting isolated chromosomes and is also useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells.

B1098 | DAPI DataSheet

Alternate Name/Synonyms: 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; 4′,6-Diamidino-2-phenylindole dihydrochloride

Appearance: Yellow solid

Formulation: N/A

CAS Number: 28718-90-3

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: Yes

Molecular Formula: C₁₆H₁₅N₅.2HCl

Molecular Weight: 350.25

Cell-Permeable?: Yes

Purity: ≥98% by HPLC

Solubilities: DMSO (>35 mg/ml)

Handling: Protect from air and light

Country of Origin: USA

Tag Line: A useful nuclear and chromosome counterstain

MDL Number: NL5995050

PubChem CID: 160166

SMILES: C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl

InChi: InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChi Key: FPNZBYLXNYPRLR-UHFFFAOYSA-N