DCL3 | Dicer-like protein 3 | AS12 2103

DCL3 | Dicer-like protein 3 | AS12 2103 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence, Uniprot: Q9LXW7, TAIR: At3g43920

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS19 4307 | anti-DCL1 | Dicer-like protein 1, rabbit antibodiesAS15 3100 | anti-DCL2 | Endoribonuclease Dicer homolog 2, rabbit antibodiesAS15 3105 | anti-DCL4 | Dicer-like protein 4, rabbit antibodiescollection of antibodies to proteins involved in microRNA metabolismPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 117 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Arabidopsis thaliana

Not reactive in: Zea mays

Additional Information: Recommend protein load is from 40-50 µg/well (using 1, 5 mm spacers helps to obtain wider wells)

Background: DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS) . Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs) . Synonymes: Endoribonuclease Dicer homolog 3.

Reconstitution: For reconstitution add 200 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT3G43920

Category: microRNA

Research Area: RNAi/siRNA/miRNA