DEDAF Antibody | 2227

DEDAF Antibody | 2227 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: DEDAF antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DEDAF.
The immunogen is located within the last 50 amino acids of DEDAF.

Research Area: Apoptosis

Tested Application: E, WB, IHC-P, IF

Application: DEDAF can be used for detection of DEDAF by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1203 - A549 Cell Lysate

Positive Control 2: Cat. No. 1211 - HepG2 Cell Lysate

Positive Control 3: Cat. No. 1404 - Mouse Liver Tissue Lysate

Positive Control 4: Cat. No. 17-003 - A549 Cell Slide

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 32 kDa

Validation: N/A

Isoform: N/A

Purification: DEDAF Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DEDAF Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DEDAF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: DEDAF Antibody: AAP1, DEDAF, YEAF1, RING1 and YY1-binding protein, Apoptin-associating protein 1, APAP-1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: DEDAF Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain (DD) death effector domain (DED) , and caspase recruitment domain (CARD) containing molecules. Several molecules including caspases and adaptor FADD contain DEDs. A novel protein that interacts with DED of caspase-8 and 10, and FADD was identified recently and designated DEDAF for DED associated factor. DEDAF is identical to the transcriptional repressor RYBP. DEDAF/RYBP is expressed in multiple tissues and cell lines. DEDAF interacts with FADD and augments the formation of CD95/FADD/capase-8 complexes at the cell membrane, and interacts with DED-containing DNA biding protein (DEDD) in the nucleus indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.