Delta-VPE | Vacuolar-processing enzyme delta-isozyme | AS20 4408

Delta-VPE | Vacuolar-processing enzyme delta-isozyme | AS20 4408 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant, His6-tagged, delta-VPE from Arabidopsis thaliana, UniProt: Q9LJX8, TAIR: At3g20210

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.

Format: Liquid at 2 mg/ml.

Tested Application: Immunofluorescence (IF), Immunolocalisation (IL), Western blot (WB)

Related Products: collection of antibodies to membrane transport system

Recommended Dilutions: 1: 500 (IF), 1: 5000 (IL), 1: 5000 (WB)

Molecular weight: 52 kDa (inactive precursor) | 37-38 kDa (mature, active form)

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus Species of your interest not listed? Contact us

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: Subcellular localisation is seed specific and restricted to developing seeds at 7 days after anthesis, Delta-VPE is detected at lower levels in flowers siliques, specifically in seed coats

Background: Delta VPE (Vacuplar-Processing Enzyme delta-isozyme) is Asparagine specific endopeptidase that may be involved in processing of proteins targeted to vacuoles (By similarity) . The enzyme is probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds (PubMed:12417707, PubMed:14688293) . Exhibits a caspase-1-like activity in extracellular granules. At the early stage of seed development, required for the formation of the seed coat, by regulating cell death of specific cell layers in inner integument (Nakaune et al. 2005) .

Reconstitution: N/A

Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: At3g20210

Category: Vacuolar membrane

Research Area: Plant Biology