DetectX® Cortisone Chemiluminescent ELISA Kit

The DetectX^® Cortisone Chemiluminescent Immunoassay (CLIA) kit is designed to quantitatively measure Cortisone present in samples. A cortisone standard is provided to generate a standard curve for the assay. After a two hour incubation the plate is washed and the chemiluminescent substrate is added. The substrate reacts with the bound cortisone-peroxidase conjugate to produce light. The generated light is detected in a multilabel microtiter plate reader.

Cortisone and cortisol concentrations vary due to the activity of two 11β-hydroxysteroid dehydrogenases (11-HSD). While most tissues have the ability to express either enzyme, 11β-HSD1 is found primarily in the liver where it converts cortisone to cortisol while 11β-HSD2 is found in tissues such as the kidney where cortisol receptor binding is required. 11β-HSD2 deactivates cortisol to cortisone, prohibiting receptor activation. This glucocorticoid “shuttle” helps to initiate and regulate the anti-inflammatory response. Monitoring the ratio of cortisone:cortisol has applications in diabetes, obesity, metabolic syndrome, osteoporosis, and chronic fatigue syndrome in addition to adrenal diseases. Cortisone and cortisol concentrations exhibit a predictable diurnal pattern and can be measured in extracted dried feces, or in serum, plasma, saliva and urine. A recent publication has suggested that salivary cortisone is a good surrogate marker for serum cortisol.

• USE - Measure the Activity of 11β-HSD Enzymes in 2 hours
• SAMPLE - Plasma, Serum, Saliva, Urine, Media, and Fecal Extracts
• SAMPLES/KIT - 37 or 229 in Duplicate
• STABILITY - Stable 4?C Liquid Reagents

DetectX® Cortisone Chemiluminescent ELISA Kit DataSheet