DetectX® Endothelin-1 (ET-1) ELISA Kit

The DetectX^® Endothelin-1 (ET-1) ELISA Kit is designed to quantitatively measure ET-1 present in a variety samples and tissue culture media. An ET-1 standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with a monoclonal antibody to capture ET-1 present in the sample. After a 60 minute incubation, the plate is washed and a peroxidase conjugated ET-1 antibody is added. The plate is again incubated for 60 minutes and washed. Substrate is then added to the plate, which reacts with the bound ET-1 conjugated antibody. After a third incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The concentration of the ET-1 in the sample is then calculated.

• Use - Measure ET-1 in Plasma, Serum and Biological Samples
• Sample - Serum, Plasma, or TCM
• Samples/Kit - 39 in Duplicate
• Sensitivity - Measures < 0.58 pg/mL ET-1
• Stability - All Reagents Stable at 4?C
• Economical - No C18 Cartridges - Supplied Extraction Solution

DetectX® Endothelin-1 (ET-1) ELISA Kit DataSheet