DFFB Antibody | 13-435

DFFB Antibody | 13-435 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 109-338 of human DFFB (NP_004393.1) .

Research Area: Apoptosis, Cell Cycle

Tested Application: WB, IHC

Application: WB: 1:200 - 1:1000
IHC: 1:50 - 1:200

Specificiy: N/A

Positive Control 1: A-549

Positive Control 2: K-562

Positive Control 3: SW620

Positive Control 4: Mouse spleen

Positive Control 5: Mouse thymus

Positive Control 6: N/A

Molecular Weight: Observed: 39kDa

Validation: N/A

Isoform: N/A

Purification: Affinity purification

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration: N/A

Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.

Alternate Name: CAD, CPAN, DFF-4DFF2, DFF4DNA fragmentation factor subunit beta, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) , caspase-activated DNase, caspase-activated deoxyribonuclease, caspase-activated nuclease

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.