Description
DFFB Antibody | 13-435 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 109-338 of human DFFB (NP_004393.1) .
Research Area: Apoptosis, Cell Cycle
Tested Application: WB, IHC
Application: WB: 1:200 - 1:1000
IHC: 1:50 - 1:200
Specificiy: N/A
Positive Control 1: A-549
Positive Control 2: K-562
Positive Control 3: SW620
Positive Control 4: Mouse spleen
Positive Control 5: Mouse thymus
Positive Control 6: N/A
Molecular Weight: Observed: 39kDa
Validation: N/A
Isoform: N/A
Purification: Affinity purification
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration: N/A
Storage Condition: Store at -20˚C. Avoid freeze / thaw cycles.
Alternate Name: CAD, CPAN, DFF-4DFF2, DFF4DNA fragmentation factor subunit beta, DNA fragmentation factor 40 kDa subunit, DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) , caspase-activated DNase, caspase-activated deoxyribonuclease, caspase-activated nuclease
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined.