DFHO | B3201

Mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. Upon binding to the Corn™ aptamer, DFHO is converted to a highly fluorescent state that can be detected at the emission wavelength of 545 nm. DFHO can also bind Broccoli™ derivative aptamers and turn on their fluorescence. DFHO is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Corn™ RNA tag. When bound to Corn™, DFHO has been found to be highly photostable compared to DFHBI (Cat. No. B2797), DFHBI-1T (B2799), and mVenus. The photostability of DFHO was demonstrated in the imaging and quantitative measurement of cellular Pol III promoter activity in living cells.

Mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. Upon binding to the Corn™ aptamer, DFHO is converted to a highly fluorescent state that can be detected at the emission wavelength of 545 nm. DFHO can also bind Broccoli™ derivative aptamers and turn on their fluorescence. DFHO is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Corn™ RNA tag. When bound to Corn™, DFHO has been found to be highly photostable compared to DFHBI (Cat. No. B2797), DFHBI-1T (B2799), and mVenus. The photostability of DFHO was demonstrated in the imaging and quantitative measurement of cellular Pol III promoter activity in living cells.

B3201 | DFHO DataSheet

Alternate Name/Synonyms: 3,5-difluoro-4-hydroxybenzylidene imidazolinone-2-oxime

Appearance: Orange solid

Formulation:

CAS Number: 1420815-34-4

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₂H₉F₂N₃O₃

Molecular Weight: 281.22

Cell-Permeable?: TRUE

Purity: ≥98% by HPLC

Solubilities: >25 mg/ml DMSO

Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.

Country of Origin: USA

Tag Line: A RFP fluorophore mimic for imaging RNA in living cells

MDL Number:

PubChem CID: 146018929

SMILES: CN1C(=CN=O)NC(=CC2=CC(=C(C(=C2)F)O)F)C1=O

InChi: InChI=1S/C12H9F2N3O3/c1-17-10(5-15-20)16-9(12(17)19)4-6-2-7(13)11(18)8(14)3-6/h2-5,16,18H,1H3/b9-4-,10-5?

InChi Key: GXOMNFVJDRZPKL-JBAXBYMVSA-N