Description
Mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. Upon binding to the Corn™ aptamer, DFHO is converted to a highly fluorescent state that can be detected at the emission wavelength of 545 nm. DFHO can also bind Broccoli™ derivative aptamers and turn on their fluorescence. DFHO is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Corn™ RNA tag. When bound to Corn™, DFHO has been found to be highly photostable compared to DFHBI (Cat. No. B2797), DFHBI-1T (B2799), and mVenus. The photostability of DFHO was demonstrated in the imaging and quantitative measurement of cellular Pol III promoter activity in living cells.
Mimic of red fluorescent protein (RFP) fluorophore for imaging RNA in living cells. Upon binding to the Corn™ aptamer, DFHO is converted to a highly fluorescent state that can be detected at the emission wavelength of 545 nm. DFHO can also bind Broccoli™ derivative aptamers and turn on their fluorescence. DFHO is cell-permeable with negligible toxicity in living cells and can be used to label any genetically encoded Corn™ RNA tag. When bound to Corn™, DFHO has been found to be highly photostable compared to DFHBI (Cat. No. B2797), DFHBI-1T (B2799), and mVenus. The photostability of DFHO was demonstrated in the imaging and quantitative measurement of cellular Pol III promoter activity in living cells.
Alternate Name/Synonyms: 3,5-difluoro-4-hydroxybenzylidene imidazolinone-2-oxime
Appearance: Orange solid
Formulation:
CAS Number: 1420815-34-4
Structure Available?: Yes
Peptide sequence: N/A
Salt Form: No
Molecular Formula: C₁₂H₉F₂N₃O₃
Molecular Weight: 281.22
Cell-Permeable?: TRUE
Purity: ≥98% by HPLC
Solubilities: >25 mg/ml DMSO
Handling: Do not take internally. Wear gloves and mask when handling the product! Avoid contact by all modes of exposure.
Country of Origin: USA
Tag Line: A RFP fluorophore mimic for imaging RNA in living cells
MDL Number:
PubChem CID: 146018929
SMILES: CN1C(=CN=O)NC(=CC2=CC(=C(C(=C2)F)O)F)C1=O
InChi: InChI=1S/C12H9F2N3O3/c1-17-10(5-15-20)16-9(12(17)19)4-6-2-7(13)11(18)8(14)3-6/h2-5,16,18H,1H3/b9-4-,10-5?
InChi Key: GXOMNFVJDRZPKL-JBAXBYMVSA-N
Additional Information
Storage Condition: |
-20ºC |
Shipping Condition: |
Gel Pack |
Shelf Life: |
36 months |