Dipeptidylpeptidase IV, human recombinant | 4710

DPP4 is a serine exopeptidase and complex enzyme that is expressed on the surface of most cell types as an intrinsic membrane glycoprotein that cleaves X-proline dipeptides from the N-terminus of polypeptides and plays a role in t-cell activation. DPP4 is associated with intracellular signal transduction, apoptosis and involved in tumor biology. There are at least 63 substrates which can bind specifically to DPP4 enzyme including growth factors, chemokines, neuro peptides. Furthermore, DPP4 plays a major role in glucose metabolism by cleaving incretins such as glucose-dependent insulinotropic polypeptide (GIP) and glucagon-like peptide-1 (GLP-1). DPP4 Human Recombinant is a single, glycosylated polypeptide chain containing 746 amino acids (aa 39-766) with a C-terminal His-tag and is purified using conventional chromatography techniques.

4710 | Activin A rat recombinant DataSheet

Biomolecule/Target: N/A

Synonyms: CD26, ADABP, ADCP2, DPPIV, TP103, DPP4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, Adenosine deaminase complexing protein 2, CD26 antigen.

Alternates names: CD26, ADABP, ADCP2, DPPIV, TP103, DPP4, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, DPP IV, T-cell activation antigen CD26, Adenosine deaminase complexing protein 2, CD26 antigen.

Taglines: An antigenic enzyme associated with immune regulation, signal transduction and apoptosis.

NCBI Gene ID #: 6387

NCBI Gene Symbol: SDF1A

Gene Source: Human

Accession #: P48061

Recombinant: Yes

Source: High-5 cells

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: <0.1 ng/g

Activity (Specifications/test method): >20 Units/mg

Biological activity: Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 20-80 ng/ml.

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit will hydrolyze 1 mmole of p-nitroaniline per minute at pH 8.0 at 37°C using 1mM of Gly-Pro p-nitroanilde as a substrate.

Molecular Weight: 8.0 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: 0.5 mg/ml; Sterile filtered solution containing 20 mM Tris-HCl buffer pH 8.0,100 mM NaCl, 1 mM EDTA and 10 % glycerol.

Reconstitution Instructions: Reconstitution in HO to a concentration of 0.1-1.0 mg/ml. The solution can then be diluted into other aqueous buffers or stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A