DISP1 Antibody | 6629

DISP1 Antibody | 6629 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: DISP1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human DISP1.
The immunogen is located within amino acids 20 - 70 of DISP1.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: DISP1 antibody can be used for detection of DISP1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of DISP1 are known to exist; this antibody will detect both isoforms. DISP1 antibody is predicted to not cross-react with EMX1.

Positive Control 1: Cat. No. 1212 - 3T3 Cell Lysate

Positive Control 2: Cat. No. 10-401 - Human Kidney Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: DISP1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DISP1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DISP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: DISP1 Antibody: DISPA, DISPA, Protein dispatched homolog 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: DISP1 Antibody: DISP1 is the mammalian homolog of the Drosophila Dispatched segment-polarity gene and a key regulator of the Hedgehog (Hh) signaling pathway during embryonic development. DISP1 is required to move Shh from site of synthesis during embryogenesis; mutations in the gene result in lethality at mid-gestation and prevents specification of ventral cell types in the neural tube. Recent results have shown that DISP1 mediates the basolateral secretion of Shh and regulates growth of mammalian long bones through the control of Ihh.