DNAJC13 Antibody | 8109

DNAJC13 Antibody | 8109 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: DNAJC13 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DNAJC13.
The immunogen is located within amino acids 1940 - 1990 of DNAJC13.

Research Area: Homeostasis

Tested Application: E, WB, IHC-P

Application: DNAJC13 antibody can be used for detection of DNAJC13 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: DNAJC13 antibody is human and mouse reactive. At least two siforms of DNAJC13 are known to exist; this antibody will detect both isoforms. DNAJC13 antibody is predicted to not cross-react with other DNAJC family members.

Positive Control 1: Cat. No. 1301 - Human Heart Tissue Lysate

Positive Control 2: Cat. No. 10-501 - Human Heart Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 249 kDa
Observed: 249 kDa

Validation: N/A

Isoform: N/A

Purification: DNAJC13 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DNAJC13 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DNAJC13 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: DNAJ homolog subfamily C member 13, receptor-mediated endocytosis 8, RME8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: DNAJC13, also known as receptor-mediated endocytosis 8 (RME8) , is the human homolog to a DnaJ domain-containing protein originally identified in a screen for endocytic defects in C. elegans (1) . It is thought to be a co-chaperone of Hsc70 which regulates protein conformation at membrane sites and plays a role in intracellular trafficking, co-localizing with markers of the endosomal system. Recent experiments have indicated that the DNAJC13 protein is involved in membrane trafficking through early endosomes but not through degradative organelles (2) . DNAJC13 has been also been shown to regulate the intracellular trafficking of the epidermal growth factor receptor (3) .