Description
DNase II Antibody | 2059 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human
Homology: N/A
Immunogen: DNase II antibody was raised against a 14 amino acid peptide near the carboxy terminus of human DNase II.
The immunogen is located within the last 50 amino acids of DNase II.
Research Area: Apoptosis, Signal Transduction
Tested Application: E, WB, IHC-P, IF
Application: DNase II antibody can be used for detection of DNase II expression by Western blot at 0.5 μg/mL. An approximate 40 kDa band can be detected, which represents the pro-enzyme of DNase II. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 1306 - Human Spleen Tissue Lysate
Positive Control 2: Cat. No. 1208 - THP-1 Cell Lysate
Positive Control 3: Cat. No. 10-901 - Human Spleen Tissue Slide
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Aprox: 40 kDa
Validation: N/A
Isoform: N/A
Purification: DNase II Antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: DNase II Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/ml
Storage Condition: DNase II antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Alternate Name: DNase II Antibody: DNL, DNL2, DNASE2A, Deoxyribonuclease-2-alpha, Acid DNase, DNase II alpha
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: DNase II Antibody: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins including AIF and Acinus. DNase II causes both chromatin condensation and DNA fragmentation. The genes encoding human, porcine, and murine DNase II have been cloned. The DNase II gene encodes a 40 kDa proenzyme. The mature enzyme consists of two non-identical subunits, the 32 kDa (alpha) and 12 kDa (beta) chains, generated by proteolytic processing. Overexpression of DNase II induces chromatin condensation. DNase II is ubiquitously expressed in human tissues.