Dnmt3L Antibody (C-term) [APR30600G]

Dnmt3L Antibody (C-term) [APR30600G] | Gentaur UK, US & Europe Distribution

Product Category: Polyclonal Antibodies

Host: Rabbit

Species Reactivity: H

Specificity: This Dnmt3L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 357-388 amino acids from the C-terminal region of human Dnmt3L.

Cellular Localisation: Nucleus.

Molecular Weight: 43583

Clone: Polyclonal

Gene Name: DNMT3L

Gene ID: 29947

Function: Catalytically inactive regulatory factor of DNA methyltransferases that can either promote or inhibit DNA methylation depending on the context (By similarity) . Essential for the function of DNMT3A and DNMT3B: activates DNMT3A and DNMT3B by binding to their catalytic domain (PubMed:17687327) . Acts by accelerating the binding of DNA and S-adenosyl-L-methionine (AdoMet) to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases (PubMed:17687327) . Recognizes unmethylated histone H3 lysine 4 (H3K4me0) and induces de novo DNA methylation by recruitment or activation of DNMT3 (PubMed:17687327) . Plays a key role in embryonic stem cells and germ cells (By similarity) . In germ cells, required for the methylation of imprinted loci together with DNMT3A (By similarity) . In male germ cells, specifically required to methylate retrotransposons, preventing their mobilization (By similarity) . Plays a key role in embryonic stem cells (ESCs) by acting both as an positive and negative regulator of DNA methylation (By similarity) . While it promotes DNA methylation of housekeeping genes together with DNMT3A and DNMT3B, it also acts as an inhibitor of DNA methylation at the promoter of bivalent genes (By similarity) . Interacts with the EZH2 component of the PRC2/EED-EZH2 complex, preventing interaction of DNMT3A and DNMT3B with the PRC2/EED-EZH2 complex, leading to maintain low methylation levels at the promoters of bivalent genes (By similarity) . Promotes differentiation of ESCs into primordial germ cells by inhibiting DNA methylation at the promoter of RHOX5, thereby activating its expression (By similarity) .

Summary: Tissue Location: Expressed at low levels in several tissues including testis, ovary, and thymus.

Form: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Application: WB, IHC-P

Dilution: WB--1:1000 IHC-P--1:100~500

Synonyms: DNA (cytosine-5) -methyltransferase 3-like, DNMT3L