Dog GDF5 (Growth Differentiation Factor 5) ELISA Kit | ELK8597

Dog GDF5 (Growth Differentiation Factor 5) ELISA Kit | ELK8597 | Gentaur UK, US & Europe Distribution

Alternative Names: CDMP1; LAP4; SYNS2; BMP14; Radotermin; Cartilage-Derived Morphogenetic Protein-1; Bone morphogenetic protein 14; Lipopolysaccharide-associated protein 4

Species: Dog

Assay Type: Sandwich

Sensitivity: 32 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Signal transduction;Neuro science;Developmental science;Bone metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Differentiation Factor 5 (GDF5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Growth Differentiation Factor 5 (GDF5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Differentiation Factor 5 (GDF5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Growth Differentiation Factor 5 (GDF5) in the samples is then determined by comparing the OD of the samples to the standard curve.