451 Recombinant Proteins and Cell culture
Donkey anti-Rabbit IgG (H&L), TRITC conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG | AS10 1019
- SKU:
- 451-AS10 1019
- Availability:
- Usually ships in 5 working days
Description
Donkey anti-Rabbit IgG (H&L), TRITC conjugated, min, cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG | AS10 1019 | Gentaur UK, US & Europe Distribution
Immunogen: N/A
Host: Donkey
Conjugation: TRITC
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified IgG.
Format: Lyophilized
Tested Application: N/A
Related Products: other fluorescent dye conjugated antibodies to rabbit IgG
Recommended Dilutions: The optimal working dilution should be determined by the investigator, (ICC), immunohistochemistry ( IHC) (Flow cyt)
Molecular weight: N/A
Confirmed Reactivity: N/A
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: N/A
Background: Donkey anti-rabbit IgG (H&L) is a Rhodamine (TRITC - tetramethylrhodamine-5-isothiocyanate) conjugated secondary antibody which binds to all rabbit IgGs in immunological assays. TRITC has Amax = 550 nm, Emax = 570 nm. Antibody is adsorbed to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, sheep IgG and affinity purified using solid phase rabbit IgG.
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: FITC, TRITC
Research Area: Immunoassays