High-quality LUV liposomes labeled with Dil are ready to use. The liposomes are prepared by the extrusion method to 100nm with very tight particle size distribution. The product is sterile filtered and filled in sterile vials guaranteed to be stable for 6 months under recommended storage conditions.

Specifications

Lipid composition: DOPC/CHOL (54:45 mol/mol)

Lipid concentration: 50 mM (50-55 mM)

DiI: 0.5mM (0.47mg/mL)

Mean particle size: 100 nm (90-120 nm)

Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.3 ± 0.2

Storage: in the refrigerator (2 – 8 degree C)

Shelf-life: 6 months guaranteed for unopened vials

DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine

CHOL: cholesterol

DiI; DiIC18(3) is a lipophilic membrane dye. It is weakly fluorescent until incorporated into liposome membranes. This orange—red-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is often used as a long-term tracer for neuronal and other cells.