High-quality LUV liposomes labeled with NBD – PE are ready to use. The liposomes are prepared by the extrusion method to 100nm with very tight particle size distribution. The product is sterile filtered and filled in sterile vials guaranteed to be stable for 6 months stored refrigerated. The phospholipid, NBD-PE is labeled on the head group with the environment-sensitive fluorophore, NBD (excitation/emission maxima ~463/536 nm).
Specifications
Product code: F60103F-NBD
Lipid composition: DOPC/CHOL/NBD-PE (54:45:1 mol/mol)
Lipid concentration: 50 mM (50-55 mM)
NBD – PE: 0.5 mM (0.50 mg/mL)
Volume: 1.0mL
Mean particle size: 100 nm (90-120 nm)
Hydration buffer: 10% sucrose, 20mM HEPES, pH 7.5 ± 0.2
Storage: in the refrigerator (2 – 8 degree C)
Stability: 6 months for unopened vials
DOPC: 1,2-dioleoyl-sn-glycero-3-phosphocholine
CHOL: cholesterol
NBD – PE: N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycerol-3-Phosphoethanolamine, Triethylammonium Salt