DPF3 Antibody | 7559

DPF3 Antibody | 7559 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Chicken: (83%)

Immunogen: DPF3 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human DPF3.
The immunogen is located within amino acids 220 - 270 of DPF3.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: DPF3 antibody can be used for detection of DPF3 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry at 5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: DPF3 antibody is human and mouse reactive. Multiple isoforms of DPF3 are known to exist.

Positive Control 1: Cat. No. 1403 - Mouse Brain Tissue Lysate

Positive Control 2: Cat. No. 10-301 - Human Brain Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 39 kDa
Observed: 50 kDa

Validation: N/A

Isoform: N/A

Purification: DPF3 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: DPF3 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: DPF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: DPF3 Antibody: CERD4, BAF45C, CERD4, Zinc finger protein DPF3, BRG1-associated factor 45C

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The DPF3 protein, also known as Cerd4, is a member of the d4 gene family of transcription modulators that also includes DPF1/Neud4 and DPF2/Requiem (1) . DPF3 has been shown to be a epigenetic key factor for heart and muscle development and can bind to methylated and acetylated lysine residues of histone 3 and 4, suggesting that DPF3 may play a role in recruiting chromatin remodeling complexes to acetylated histones (2) . Two isoforms of DPF3, DPF3a and DPF3b, are required as transcriptional co-activators in SWI/SNF complex-dependent activation of the NF-kappaB RelA/p50 heterodimer (3) .