DR6 Antibody [Luke-1] | XA-1015

DR6 Antibody [Luke-1] | XA-1015 | Gentaur UK, US & Europe Distribution

Host: Rat

Reactivity: Human

Homology: N/A

Immunogen: DR6 (monoclonal Luke-1) antibody was raised against recombinant human DR6;Fc (aa. 42-335) .

Research Area: Apoptosis

Tested Application: Flow

Application: DR6 antibody can be used to recognize human DR6 by Flow Cytometry. Method: Cells (5x105) were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% FCS, 0.02% azide) containing 1 μg/mL of anti-DR6 MAb (Luke-1) . After washing in FACS buffer, R-PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and analyzed by flow cytometry. Method: 3 HEK 293T cells (5 x 105) were mock transfected (thin line) or transfected with an expression plasmid enabling surface expression of mDR6 (thick line) . Cells were incubated on ice for 30 min in 50 μl FACS buffer (PBS, 5% Fetal calf serum, 0.02% azide) containing 1 μg/mL of Luke-1 antibody. After washing in FACS buffer, PE-conjugated antibody to rat IgG was added. Cells were incubated on ice for 30 min, washed and then analyzed by flow cytometry.

Specificiy: N/A

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: DR6 (monoclonal Luke-1) antibody was purified to ≥95% in SDS-PAGE.

Clonality: Monoclonal

Clone: Luke-1

Isotype: IgG2a

Conjugate: Unconjugated

Physical State: Liquid

Buffer: PBS containing 0.02% sodium azide

Concentration: N/A

Storage Condition: DR6 antibody can be stored at 4˚C, stable for one year.

Alternate Name: N/A

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: N/A