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Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base | MBS652948

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SKU:: MBS652948-GEN
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Description

Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base, Culture Media

Full Product Name: Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base (Powder)

Product Synonym Names: Synthetic complete amino acid supplement-minus uracil (UraDO)

Research Use Only: For Research Use Only. Not for use in diagnostic procedures.

Purity/Purification: Molecular Biology Grade

Form/Format: White, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: 4.2

Directions per Liter (Broth): Mix 1.92 grams of drop-out mix with 6.7g MBS653287 Yeast Nitrogen Base w/o AA, Carbohydrate & w/ AS per liter of ddH2O. Adjust to desired pH. Sterilize with 0.2 um membrane filter. Aliquot into sterile containers.

Directions per Liter (Plates): Mix 1.92 grams of drop-out mix with 43.7 g MBS653105 Drop-out Base (w/o aa) With Glucose and Agar per liter of ddH2O. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121°C (15 psi).

Formulation:

Each bottle is a homogenous mixture of the following amino acids and nucleotide bases minus uracil:

Amino Acids:
p-Aminobenzoic acid: 0.2g
Alanine: 2.0g
Arginine: 2.0g
Asparagine: 2.0g
Aspartic acid: 2.0g
Cysteine: 2.0g
Glutamic acid: 2.0g
Glutamine: 2.0g
Glycine: 2.0g
Histidine: 2.0g
Isoleucine: 2.0g
Leucine: 10.0g
Lysine: 2.0g
Methionine: 2.0g
Phenylalanine: 2.0g
Proline: 2.0g
Serine: 2.0g
Threonine: 2.0g
Tryptophan: 2.0g
Tyrosine: 2.0g
Valine: 2.0g

Nucleotide Bases:
Adenine: 0.5g

Vitamins:
Inositol: 2.0g
Uracil: Absent

Use 1.92g/liter
100g makes ~50 liters

Preparation and Storage: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Other Notes: Small volumes of Drop-out Mix Synthetic Minus Uracil w/o Yeast Nitrogen Base culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

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