DSCAM Antibody | 45-499

DSCAM Antibody | 45-499 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog

Immunogen: The immunogen for this antibody is: C-DSWDSAQRTKDVSPQ

Research Area: Cancer

Tested Application: E, WB, IF, Flow

Application: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 200-220kDa band observed in HepG2 cell lysates (calculated MW of 174kDa according to NP_996770.1 ) . The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 2-3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the nuclei of MCF7 cells. Recommended concentration: 10ug/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognise both reported isoforms (NP_001380.2 and NP_996770.1) .

Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: DSCAM, Down syndrome cell adhesion molecule, CHD2-42, CHD2-52, human CHD2-52 down syndrome cell adhesion molecule

User Note: N/A

BACKGROUND: N/A