DSP Antibody | 42-801

DSP Antibody | 42-801 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human

Homology: Expected Species Reactivity based on sequence homology: Mouse, Rat

Immunogen: The immunogen for this antibody is: C-QESVKQAHLRNEH

Research Area: Other

Tested Application: E, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Preliminary experiments in lysates of cell lines HEK293, HeLa and NIH3T3 gave no specific signal but low background (at antibody concentration up to 1ug/ml) . We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Heart shows staining of the junctons between cardiomuscular fibres. Recommended concentration, 3-6ug/ml.

Specificiy: This antibody is expected to recognize reported isoform I (NP_004406.2) only.

Positive Control 1: Cat. No. 1210 - 293 Cell Lysate

Positive Control 2: Cat. No. 1201 - HeLa Cell Lysate

Positive Control 3: Cat. No. 1282 - 3T3 (NIH) Cell Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20��C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: DSP, desmoplakin, DPI, 250kDa paraneoplastic pemphigus antigen, desmoplakin I

User Note: N/A

BACKGROUND: N/A