Dynactin Antibody | 45-508

Dynactin Antibody | 45-508 | Gentaur UK, US & Europe Distribution

Host: Goat

Reactivity: Human, Mouse

Homology: N/A

Immunogen: The immunogen for this antibody is: C-QEQLHQLHSRLIS

Research Area: Cancer

Tested Application: E, WB, Flow, IF, ICC, IHC

Application: Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 150kDa band observed in Human Cerebellum lysates (calculated MW of 142kDa according to NP_004073.2) . Additional bands between approx. 40-70kDa were consistently observed, and all were successfully blocked by incubation with the immunizing peptide. Recommended concentration 1-2ug/ml. Primary incubation 1 hour at room temperature. Preliminary testing was unsuccessful on Rat Brain and Testis for this particular batch.Immunohistochemistry:In paraffin embedded Human Cerebellum shows pixulate staining of cytoplasm in neuronal cells. Recommended concentration: 2-4ug/ml. A customer found positive results in IHC on Human HeLa cells.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa and U2OS cells. Recommended concentration: 10ug/ml. Immunocytochemsitry: A customer found positive results in ICC on Mouse embryonic fibroblasts (MEF cells) .

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Specificiy: This antibody is expected to recognise all reported isoforms.

Positive Control 1: Cat. No. 1313 - Human Testis Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.

Concentration: 500 ug/mL

Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.

Alternate Name: DCTN1, dynactin 1 (p150, glued homolog, Drosophila) , P135, DP-150, DAP-150, p150-glued, p150, Glued (Drosophila) homolog, 150 kDa dynein-associated polypeptide, dynactin 1 (p150, Glued (Drosophila) homolog) , HMN7B, OTTHUMP00000202491, dynactin 1, p150, Glued homolog

User Note: N/A

BACKGROUND: N/A