E2F-1 (4G8) monoclonal Antibody | MB0176

E2F-1 (4G8) monoclonal Antibody | MB0176 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Human,Rat

Application: WB IF IP

Application Range: WB: 1:500 IP: 1:50~200 IF: 1:50~200

Background: The E2F transcription factors are essential for regulation of the cell cycle. Physiological E2F is a heterodimer composed of an E2F subunit together with a DP subunit. Six members of the E2F family have been identified, and each E2F subunit has a DNA binding and a dimerization domain. E2F-1 to -5 activate transcription. E2F-1 to -3 bind pRb, and E2F-4 and -5 bind p107 or p130, and these interactions are under cell cycle control. E2F-1 has oncogenic properties in vivo and in vitro. E2F-1 can induce apoptosis through p53-dependent and -independent mechanisms. E2F-1 is stress-responsive, and is regulated by a PI3-kinase-like kinase family such as the ATM/ATR kinases.

Storage & Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: This Antibody detects endogenous levels of E2F-1 and does not cross-react with related proteins.

Molecular Weight: Predicted band size:47KDa Observed band size:70KDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1; E2F1; RBBP3

Immunogen: Purified recombinant human E2F-1 protein fragments expressed in E.coli.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: The Antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE) .

Pathway: Mitochondrial Control of Apoptosis,