EBI3 Antibody | 4977

EBI3 Antibody | 4977 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-EBI-3 antibody (4977) was raised against a peptide corresponding to 16 amino acids near the center of mature human EBI-3.
The immunogen is located within amino acids 150 - 200 of EBI3.

Research Area: Immunology

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5 - 2 μg/mL; IF: 20 μg/mL ; IHC: 2.5 μg/mL;.
Antibody validated: Western Blot in human and rat samples; Immunofluorescence and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1466 - Rat Spleen Tissue Lysate

Positive Control 2: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 25kD
Observed: 30 kD (25kD + 2 N-Glycosylations)

Validation:

Recombinant Protein Test (Figure 2) shows that anti-IL-EBI-3 antibody (4977) detects human recombinant IL-17 (IL-17A + IL-17B) protein that contains the amino acids of 4977 antigenic peptide.

Isoform: Human EBI-3 has only 1 isoform (229aa, 25kD) . Mouse EBI-3 has one isoform (228aa, 25kD) and rat EBI-3 also has one isoform (228aa, 25kD) . 4977 can detect human isoform and possibly detect mouse and rat EBI-3.

Purification: EBI3 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: EBI3 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: EBI3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: EBI3 Antibody: IL27B, IL-27B, IL27B, Interleukin-27 subunit beta, Epstein-Barr virus-induced gene 3 protein, IL-27 subunit beta

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: EBI3 Antibody: EBI3 is a subunit in two distinct heterodimeric cytokines: interleukin-27 (IL-27) and IL-35. Like interleukin-23 (IL-23) , IL-27 is a recently discovered member of the IL-6/IL-12 family of proinflammatory and immunoregulatory cytokines. It exists as a heterodimer composed of the p40-related protein EBI3 and an IL-12 p35-related protein termed p28. IL-27 is produced after activation by antigen-presenting cells and induces proliferation of naïve but not memory CD4+ T-cells. It acts by binding to its receptor WSX-1 (also known as TCCR) and gp130 which results in the activation of a Jak/STAT signaling cascade, suggesting the IL-27 is involved in the regulation of immune processes. It has been suggested that IL-27 can also be used as a therapeutic agent against cancer as it can also induce tumor-specific anti-tumor activity mediated through CD8+ T-cells, IFN-gamma, and T-bet. IL-35 is composed of EBI3 and the p35 subunit of IL-12 and has been reported to have therapeutic effects against collagen-induced arthritis by expanding the population of regulatory T cells and suppressing Th17 cells. At least two isoform of EBI3 are known to exist.