ECHS1, human recombinant | P1191

Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

P1191 | ECHS1 human recombinant DataSheet

Biomolecule/Target: ECHS1

Synonyms: Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH, Enoyl-CoA hydratase 1, Short-chain enoyl-CoA hydratase

Alternates names: Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN- 2a

Taglines: Member of the hydratase/isomerase superfamily that funtions in the mitochondrial fatty acid beta-oxidation pathway.

NCBI Gene ID #: 3440

NCBI Gene Symbol: IFNA2

Gene Source: Human

Accession #: P01563

Recombinant: Yes

Source: E.coli

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.

Biological activity: The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1 x 10 IU/mg

Results: > 150 units/mg

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C

Molecular Weight: 19.4 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol, 100 mM NaCl

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A