26
ECHS1, human recombinant | P1191
- SKU:
- 26-P1191-GEN
- Availability:
- Usually shipped in 5 working days
Description
Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, also known as ECHS1, is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Expressed in muscle, liver and fibroblasts, with low expression in kidney and spleen, ECHS1 exists as a homohexamer that functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. Recombinant human ECHS1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
P1191 | ECHS1 human recombinant DataSheet
Biomolecule/Target: ECHS1
Synonyms: Enoyl Coenzyme A hydratase, short chain 1 mitochondrial, SCEH, Enoyl-CoA hydratase 1, Short-chain enoyl-CoA hydratase
Alternates names: Leukocyte interferon, B cell interferon, Type I interferon, IFNA2, IFN- 2a
Taglines: Member of the hydratase/isomerase superfamily that funtions in the mitochondrial fatty acid beta-oxidation pathway.
NCBI Gene ID #: 3440
NCBI Gene Symbol: IFNA2
Gene Source: Human
Accession #: P01563
Recombinant: Yes
Source: E.coli
Purity by SDS-PAGEs: 95%
Assay: SDS-PAGE
Purity: N/A
Assay #2: N/A
Endotoxin Level: N/A
Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C.
Biological activity: The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1 x 10 IU/mg
Results: > 150 units/mg
Binding Capacity: N/A
Unit Definition: One unit is defined as the amount of enzyme that hydrolyze 1.0 umole of crotonoyl-CoA to hydroxybutyryl-CoA per minute per minute at pH 7.5 at 25C
Molecular Weight: 19.4 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM DTT, 20% glycerol, 100 mM NaCl
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1 mg/ml. This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A