EDA1 Antibody | 8045

EDA1 Antibody | 8045 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (89%)

Immunogen: EDA1 antibody was raised against an 19 amino acid peptide near the center of human EDA1.
The immunogen is located within amino acids 130 - 180 of EDA1.

Research Area: Stem Cell

Tested Application: E, WB, IHC-P, IF

Application: EDA1 antibody can be used for detection of EDA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.

Specificiy: EDA1 antibody is human, mouse and rat reactive. Multiple isoforms of EDA1 are known to exist.

Positive Control 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 16, 43 kDa
Observed: 39 kDa

Validation: N/A

Isoform: N/A

Purification: EDA1 antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: EDA1 antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: EDA1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Ectodysplasin A, Ectodermal dysplasia protein, ED1, ED1-A1, ED1-A2, EDA1, EDA2, EDA protein, HED, ODT1, STHAGX1, XHED, XLHED

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Ectodysplasin A (EDA1) is a member of the TNF-related ligand family involved in the early epithelial-mesenchymal interaction that regulates ectodermal appendage formation (1) . It is a trimeric type II membrane protein that co-localizes with cytoskeletal structures at the lateral and apical surfaces of cells and can be expressed as eight alternatively spliced isoforms in hair follicles and in the epidermis of adult skin (2, 3) . EDAs are required during development, and loss or mutation of EDA1 results in a group of developmental disorders identified as ectodermal dysplasia type 1 (4, 5) .