Description
EIF2S3 Antibody | 30-144 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat, Dog, Zebrafish
Homology: N/A
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF2S3.
Research Area: Immunology
Tested Application: E, WB
Application: EIF2S3 antibody can be used for detection of EIF2S3 by ELISA at 1:312500. EIF2S3 antibody can be used for detection of EIF2S3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.
Specificiy: N/A
Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: 51 kDa
Validation: N/A
Isoform: N/A
Purification: Antibody is purified by protein A chromatography method.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration: batch dependent
Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF2S3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Alternate Name: EIF2S3, EIF2, EIF2G, EIF2gamma, eIF-2gA
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B.