EIF4A2 Antibody | 29-278

EIF4A2 Antibody | 29-278 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat, Dog

Homology: N/A

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4A2.

Research Area: Other

Tested Application: E, WB

Application: EIF4A2 antibody can be used for detection of EIF4A2 by ELISA at 1:312500. EIF4A2 antibody can be used for detection of EIF4A2 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50, 000 - 100, 000.

Specificiy: N/A

Positive Control 1: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 45 kDa

Validation: N/A

Isoform: N/A

Purification: Antibody is purified by peptide affinity chromatography method.

Clonality: Polyclonal

Clone: N/A

Isotype: N/A

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Concentration: batch dependent

Storage Condition: For short periods of storage (days) store at 4˚C. For longer periods of storage, store EIF4A2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.

Alternate Name: EIF4A2, DDX2B, EIF4A, EIF4F, BM-010, eIF4A-II, eIF-4A-II

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: EIF4A2 contains 1 helicase C-terminal domain and 1 helicase ATP-binding domain. It belongs to the DEAD box helicase family. eIF4A subfamily. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5' untranslated region of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.