EIN3 | Ethylene insensitive 3 | AS19 4273

EIN3 | Ethylene insensitive 3 | AS19 4273 | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant EIN3 of Arabidopsis thaliana, UniProt: O24606-1, TAIR: At3g20770

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Serum

Format: Lyophilized

Tested Application: Western blot (WB)

Related Products: AS12 1865 | Anti-EIN2 | Ethylene insensitive 2, rabbit antibodiesAS06 193 | Anti-IAA | indole 3 acetic acid, rabbit antibodiesAS11 1749 | IAA | auxin ELISA quantitation kitantibodies to proteins involved in ethylene metabolismSecondary antibodies

Recommended Dilutions: 1 : 1000 (WB)

Molecular weight: 71, 4 | 78 kDa

Confirmed Reactivity: Arabidopsis thaliana

Predicted Reactivity: N/A

Not reactive in: Medicago truncatula

Additional Information: Western blot condtions for anti-EIN3 antibodies are described in Smalle et al. 2002.Recommended membrane for blocking: nitrocellulose, blocking reagent: 10 % non-fat milk, antibody incubation buffer: PBS-T.

Background: EIN3 (Ethylene insensitive 3) acts as a positive regulator in the ethylene response pathway and is required for ethylene responsiveness in adult plant tissues. Activated by phosphorylation by MPK3 and MPK6 (PubMed:18273012) . Down-regulated by KIN10 that controls its protein stability under a phosphorylation-dependent manner. Alternative names: Protein ethylene insensitive 3.

Reconstitution: For reconstitution add 50 µl, of sterile water

Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: At3g20770

Category: Ethylene

Research Area: Plant Biology