Emi1 polyclonal Antibody | BS90461

Emi1 polyclonal Antibody | BS90461 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Application: WB ICC IHC

Application Range: ICC:1:50-1:200 IHC:1:50-1:200 WB:1:500

Background: Emi1 (for early mitotic inhibitor) regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC) . Emi1 is a conserved F box protein containing a zinc binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb) . Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry. Emi1 is required to arrest unfertilized eggs at metaphase of meiosis II and may be the long-sought mediator of CSF activity. Human Emi1 is similar to Xenopus laevis Emi1, which inhibits the APC (Cdc20) ubiquitination complex to allow accumulation of cyclin B.

Storage & Stability: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.

Specificity: Emi1 polyclonal Antibody detects endogenous levels of Emi1 protein.

Molecular Weight: 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Alternative Names: FBXO5, EMI1, FBX5, Early mitotic inhibitor 1, Emi 1, EMI1, F box only protein 5, F box protein 5, F box protein Fbx 5, F box protein Fbx5, F-box only protein 5, FBX5, FBX5, Fbxo31, fbxo5,

Immunogen: Recombinant protein within human Emi1 aa 50-250.

Conjugate: Unconjugated

Modification: Unmodification

Purification & Purity: ProA affinity purified

Pathway: