ENA ELISA kit | 55R-ORG506

ENA ELISA kit | 55R-ORG506 |

Activity Code: ACTIVE

Product Type: Research Kits

Product Subtype: Kits

Research Area: DNA & RNA

Short Description: ELISA kit for the detection of ENA in the research laboratory

Immunogen: N/A

Host: N/A

Specificity: N/A

Cross Reactivity: N/A

Isotype: N/A

IClone: N/A

Species: N/A

Residues: N/A

Tag/conjugate: N/A

Protein Type: N/A

Expression System: N/A

Grade & Purity: N/A

Methode of Purification: N/A

Source: N/A

Concentration: N/A

Form & Buffer: N/A

Storage: Store at 2-8 deg C

Shipping Information: *Blue Ice*

Applications: ELISA

Bioactivity: N/A

Usage Recommendations: Optimal conditions to be determined by end-user

Assay Information: A mixture of extractable nuclear antigens (SSA (Ro), SSB (La), Sm, RNP/Sm, Scl 70 and Jo1) are bound to microwells. Antibodies against these antigens, if present in diluted serum or plasma, bind to the respective antigens. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically detects the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.